Clathrin, Light Chain A/B (CLTA/CLTB) antibody

Antigen: Clathrin, Light Chain A/B (CLTA/CLTB)

Synonyms: LCA, AV026556, CLTA, CLTA2, MGC140767, lca, MGC75712, zgc:73358, wu:fb61d08, wu:fj48d06, CLTB, clc, clcb

Clonality: Monoclonal (CON-1)

Host: Mouse

Reactivity: Cow (Bovine), Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN180616

Additional Information

Characteristics: Synonyms: Lca, CLTA

Alternative name: Clathrin light chain A (CLTA/CLTB)

Gene ID: 281078

Swiss-Prot: P04973

Immunogen: Synthetic peptide derived from bovine clathrin light chain corresponding to the conservedregion of the light chains, residues 23-44. Remarks: Mol. Wt. of Antigen: 25-29 kDa

Isotype: IgG2b  (Matching secondary antibodies)

Clone: CON-1

Description: Clathrin consists of three heavy chain (192 kDa) and three light chains (LCa or LCb,25-29 kDa) and self-assembles into a polyhedral cytoskeletal element. Self-assemblyfacilitates clathrin's central role in membrane sorting and selective transport within thecell.

Specificity: This antibody detects Clathrin. It reacts with both LCa and LCb. Species: Human, Cow. Others not tested. Add. Information: Cellular Localization: Cell membrane and cytoplasm

Application Details

Application Notes: Immunofluorescence. Immunoprecipitation (Denatured verified): use Protein A, 2 g/mg protein. Western Blotting (2): 1-2 ug/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): 1-2 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: Placenta. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.

Storage: Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only