Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (AUA1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN180642

Additional Information

Characteristics: Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

Alternative name: CD326 / EPCAM / TACSTD1

Swiss-Prot: P16422

Immunogen: Colon carcinoma LoVo cell line. Remarks: Mol. Wt. of Antigen: 40-43 kDa

Isotype: IgG1  (Matching secondary antibodies)

Clone: AUA1

Description: EGP40 is a 40 kDa transmembrane epithelial glycoprotein, also identified as epithelialspecific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressedon baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Itreportedly distinguishes adenocarcinomas from pleural mesotheliomas.

Specificity: This antibody detects Epithelium specific antigen. Cellular Localization: Cell membraneSpecies: Human. Others not tested.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: Immunohistology (Formalin/Paraffin): 1-2 µg/ml for 30 min at RT. Staining ofFormalin/Paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1 mg/mlTris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Positive Control: Breast carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10 mM PBS, pH 7.4 containing 0.2% BSA as stabilizer and 0.09% SodiumAzide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human breast carcinoma stained with ESA (ABIN180642) using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.