Microphthalmia-Associated Transcription Factor (MITF) (N-Term) antibody

Details for Product No. ABIN180672
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Antigen
Epitope
N-Term
(19), (15), (11), (9), (8), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human
(105), (51), (23), (14), (14), (14), (2), (2), (2), (1)
Host
Mouse
(77), (28), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(86), (34), (22), (18), (17), (13), (11), (10), (10), (6), (4), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN180672
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Immunogen N-terminal fragment of human Mi protein
Clone D5
Isotype IgG1
Specificity This antibody detects MiTF. It does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay (1). Reacts with both melanocytic and nonmelanocytic isoforms of Mi.
Characteristics Synonyms: Microphthalmia-associated transcription factor, Mi-protein
Purification Protein G chromatography
Alternative Name MITF
Background Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells.There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart (1).Synonyms: Mi-protein, Microphthalmia-associated transcription factor
Gene ID 4286
UniProt O75030
Research Area Transcription Factors
Application Notes Western Blotting (Not verified). Immunohistology (Formalin/paraffin): 4-8 μg/mL for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: 501 Mel cells (1). Melanoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Microphthalmia-Associated Transcription Factor (MITF) (N-Term) antibody anti-Microphthalmia-Associated Transcription Factor (MITF) (N-Term) antibody
Background publications Hemesath, Price, Takemoto et al.: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." in: Nature, Vol. 391, Issue 6664, pp. 298-301, 1998 (PubMed).

Weilbaecher, Hershey, Takemoto et al.: "Age-resolving osteopetrosis: a rat model implicating microphthalmia and the related transcription factor TFE3." in: The Journal of experimental medicine, Vol. 187, Issue 5, pp. 775-85, 1998 (PubMed).

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