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Keratin 20 (KRT20) antibody

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(248), (53), (44), (9), (4), (2), (1)
(122), (115), (18), (2)
Clonality (Clone)
Monoclonal ()
(13), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(170), (95), (67), (59), (31), (26), (12), (11), (11), (8), (4), (4), (3), (3), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mg
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Immunogen Partially purified human keratin 20 expressed in bacterial cellsRemarks: Mol. Wt. of Antigen: 46 kDa
Clone Q6
Isotype IgG1
Characteristics Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT, Keratin type I cytoskeletal 20
Purification Protein G chromatography
Alternative Name Cytokeratin 20 (KRT20 Antibody Abstract)
Background Keratin 20 / cytokeratin20 (CK20) is a Type-I keratin which is primarily expressed in gastricand intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed inadenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is alsopresent in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably,the squamous cell carcinomas and adenocarcinomas of the breast, lung, andendometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack CK20.
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Immunoprecipitation: use protein G, 2 g/mg protein lysate. Western Blotting: 1-2 ug/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): 1-2 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HT29 cells. Colon carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Calnek, Quaroni: "Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 95-104, 1993 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Löwe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Catalog No. ABIN180683

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