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Antibodies
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anti-Microtubule Associated Protein 2 (MAP2) antibody
Microtubule Associated Protein 2 (MAP2) antibody
| Antigen |
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| Synonyms |
MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864 |
| Clonality |
Monoclonal (AP18) |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Rat (Rattus), Xenopus laevis
Human (29), Mouse (Murine) (20), Rat (Rattus) (16), Cow (Bovine) (14), Pig (Porcine) (8), Chicken (6), Xenopus laevis (4), Cat (Feline) (3), Bird (Avian) (1), Primate (1), Rabbit (1)
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Application
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Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (27), Immunofluorescence (IF) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (13), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (4), ELISA (3), ELISA (Detection) (2), Radioimmunoassay (RIA) (2)
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| Catalog no. |
ABIN180702 |
| Quantity |
0.1 mg (0.2 mg/ml) |
| Price |
510.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker
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Alternative name
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MAP-2
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Gene ID
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4133
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Swiss-Prot
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P11137
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Immunogen
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Bovine brain microtubule associated protein 2Remarks: Mol. Wt. of Antigen: ~280 kDa Doublet and a 70 kDa singlet
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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AP18
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Description
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Microtubule Associated Protein 2 exists in two high molecular weight forms (2a & 2b) and alow molecular weight form. The expression of MAP2 is developmentally regulated and itsmultiple forms arise by alternative splicing of a single gene.
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Specificity
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This antibody is specific to phosphprylated form of MAP2a,b,c and shows nocross-reaction with other MAPs, tau, and tubulin. It reacts with dendrites and cell bodies ofneurons. It is useful in studies of neuron structure in normal and malignantbrain tissue andin Alzheimer's. Species: Human, Cow, Rat, Xenopus, Quail. Others not tested. Add. Information: Cellular Localization: CytoplasmicEpitope: aa 1-151
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Application Notes
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Immunofluorescence. Western Blotting (1,2): 1-2 ug/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): 1-2 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: IMR-5 or T98G human glioblastoma cells or brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml
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Buffer
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10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
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Storage
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Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Cytoskeleton
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Restrictions
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For Research Use only
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Alternatives for antigen "Microtubule Associated Protein 2 (MAP2)", type "Antibodies"
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Hosts
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Mouse (22), Rabbit (11), Chicken (3)
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Reactivities
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Human (29), Mouse (Murine) (20), Rat (Rattus) (16), Cow (Bovine) (14), Pig (Porcine) (8), Chicken (6), Xenopus laevis (4), Cat (Feline) (3), Bird (Avian) (1), Primate (1), Rabbit (1)
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Applications
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Western Blotting (WB) (27), Immunofluorescence (IF) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (13), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (4), ELISA (3), ELISA (Detection) (2), Radioimmunoassay (RIA) (2)
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Conjugates
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Biotin (2), FITC (2), Alexa Fluor 488 (1)
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Epitopes
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C-Term (1)
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