Bone Morphogenetic Protein 4 (BMP4) antibody

Antigen: Bone Morphogenetic Protein 4 (BMP4)

Synonyms: ZYME, BMP2B, OFC11, BMP2B1, MCOPS6, Bmp-4, Bmp2b, Bmp2b1, Bmp2b-1, BOMPR4A, bmp-4, zbmp-4, MGC100779, zgc:100779, BMP4, BMP-4, xbmp4, XBMP-4, Bmp4, MGC127497, zyme, bmp2b, bmp2b1, bmp4

Clonality: Monoclonal (3H2)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN180726

Additional Information

Characteristics: Synonyms: BMP2B, BMP-2B, BMP-4, DVR4, Bone morphogenetic protein 4

Alternative name: BMP4

Swiss-Prot: P12644

Immunogen: Recombinant Human BMP-4 expressed in E. coli. Genename: BMP-4

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 3H2

Description: BMP-4 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one ofa growing number of osteogenic proteins shown to induce bone and cartilage formationand to play an important role in developmental processes, including cell proliferation,differentiation, apoptosis and morphogenesis. BMPs act through binding with a receptor complex consisting of type I and type IIserine/threonine kinases, resulting ultimately in the activation of the Smad protein andmitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins,including, noggin, chordin, gremlin and follistatin, are responsible for modulating thesignaling effects of BMPs, through the binding and blocking of receptor ligands, therebypreventing activation. Research studies have verified the particular importance of BMP-4 inepithelial-mesenchymal interactions, including its vital role in the early stages of toothmorphogenesis (3), the interaction between BMP-4 and its antagonist noggin in hairfollicles (4) and the effects of BMP-4 expression/signaling in organs, including the lungs(5) and eyes (6).

Specificity: MCA2453 recognizes the disulphide-linked homodimeric cysteine knot protein known ashuman Bone Morphogenetic Protein 4 (BMP-4/BMP-2b), full-length 408aa. Species: Human. Others not tested.

Application Details

Application Notes: ELISA: 1.0 - 4.0 ug/ml. Western Blot: 0.25ug/ml non-reducing conditions, MCA2453 detects a band ofapproximately 44kDa due to glycosylation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.2 - containing 0.09% Sodium Azide

Storage: Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.

Research Area: Organogenesis, Growth Factors, Angiogenesis, Signaling

Restrictions: For Research Use only