Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

Details for Product No. ABIN180896
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Antigen
Synonyms MKI67, kia, mki67, MGC132156, D630048A14Rik, Ki-67, Ki67, KIA, MIB-1
Reactivity
Human
(200), (30), (28), (2), (1), (1)
Host
Mouse
(125), (73), (6), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
(98), (69), (65), (57), (33), (29), (16), (16), (10), (8), (2), (1), (1)
Pubmed 7 references available
Quantity 2 mL
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Catalog No. ABIN180896
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Immunogen Human tumour cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag8. 653 myeloma cell line.
Clone Ki67
Isotype IgG1
Specificity This antibody reacts with a nuclear antigen that is expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (Go) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (Immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidinelabelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong.
Characteristics Synonyms: Ki67 antigen, MKI67, Proliferation marker
Purification Supernatant
Alternative Name Ki-67
Background Ki-67 is a nuclear protein that is expressed in proliferating cells and may be required for maintaining cell proliferation. Ki-67 has been used as a marker for cell proliferation of solid tumors and some hematological malignancies. A correlation has been demonstrated between Ki-67 index and the histopathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors.Synonyms: Ki67 antigen, MKI67, Proliferation marker
Gene ID 4288
UniProt P46013
Research Area Proliferation Markers, Cell Cycle
Application Notes Immunofluorescence. Flow Cytometry: Use 10 μL of Neat-1/5 diluted antibody to label 10^6 cells in 100 μL (Cellpermeabilisation is required). Immunohistochemistry on Frozen Sections: 1/10-1/50. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody
Background publications Houmand, Abrahamsen, Tinggaard Pedersen: "Relevance of Ki-67 expression in the classification of non-Hodgkin's lymphomas: a morphometric and double-immunostaining study." in: Histopathology, Vol. 20, Issue 1, pp. 13-20, 1992 (PubMed).

Freedman, Nadler: "Immunologic markers in non-Hodgkin's lymphoma." in: Hematology/oncology clinics of North America, Vol. 5, Issue 5, pp. 871-89, 1991 (PubMed).

Grogan, Miller: "New biologic markers in non-Hodgkin's lymphomas." in: Hematology/oncology clinics of North America, Vol. 5, Issue 5, pp. 925-33, 1991 (PubMed).

Hall, Richards, Gregory et al.: "The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma." in: The Journal of pathology, Vol. 154, Issue 3, pp. 223-35, 1988 (PubMed).

Grogan, Lippman, Spier et al.: "Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67." in: Blood, Vol. 71, Issue 4, pp. 1157-60, 1988 (PubMed).

Gerdes, Lemke, Baisch et al.: "Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1710-5, 1984 (PubMed).

Gerdes, Schwab, Lemke et al.: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, 1983 (PubMed).

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