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Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN180933, Supplier: Log in to see
Antigen
  • AGGRUS
  • E11
  • GP36
  • Gp38
  • GP40
  • HT1A-1
  • OTS-8
  • OTS8
  • PA2.26
  • PDPN
  • RANDAM-2
  • RTI40
  • T1-alpha
  • T1A
  • T1a
  • T1A-2
  • T1alpha
Reactivity
Human
245
144
29
3
Host
Mouse
103
99
97
40
27
13
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Podoplanin antibody is un-conjugated
23
18
16
13
11
11
10
9
9
9
9
9
9
8
8
8
6
6
5
4
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
281
170
126
85
84
62
53
49
38
11
4
3
3
3
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen ORF Human podoplanin ectodomain expressed in E. coliPage 1 of 3.
Clone 4D5aE5E6
Isotype IgG1
Specificity This antibody recognizes Human Podoplanin (gp36).
Characteristics Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025
Purification Affinity chromatography on Protein G
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Podoplanin a 36 kDa type I cell membrane-associated glycoprotein, with a broad spectrum of distribution, being strongly expressed on the lymphatic endothelium found in lung, placenta and skeletal muscle and to a lesser extent in brain, kidney and liver. Podoplanin is absent on blood endothelium and therefore This antibody is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumour lymphangiogenesis.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Gene ID 10630
UniProt Q86YL7
Application Notes Immunohistochemistry on frozen sections: 0.75g/mL - 2g/mL. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Recommended Positive Control: Normal lung
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS containing 0,09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Matsui, Nagy-Bojarsky, Laakkonen, Krieger, Mechtler, Uchida, Geleff, Kang, Johnson, Kerjaschki, Kang: "Lymphatic microvessels in the rat remnant kidney model of renal fibrosis: aminopeptidase p and podoplanin are discriminatory markers for endothelial cells of blood and lymphatic vessels." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 8, pp. 1981-9, 2003 (PubMed).

Dadras, Paul, Bertoncini, Brown, Muzikansky, Jackson, Ellwanger, Garbe, Mihm, Detmar: "Tumor lymphangiogenesis: a novel prognostic indicator for cutaneous melanoma metastasis and survival." in: The American journal of pathology, Vol. 162, Issue 6, pp. 1951-60, 2003 (PubMed).

Schacht, Ramirez, Hong, Hirakawa, Feng, Harvey, Williams, Dvorak, Dvorak, Oliver, Detmar: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Kato, Fujita, Kunita, Sato, Kaneko, Osawa, Tsuruo: "Molecular identification of Aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51599-605, 2003 (PubMed).

Zimmer, Oeffner, Von Messling, Tschernig, Gröness, Klenk, Herrler: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).