Interleukin 10 (IL10) antibody

Details for Product No. ABIN181026
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Antigen
Synonyms CSIF, GVHDS, IL-10, IL10A, TGIF, Il-10, IL10X
Reactivity
Human
(242), (143), (52), (26), (12), (12), (12), (11), (2), (1), (1), (1)
Host
Rat
(149), (136), (87), (6), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(63), (16), (11), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(242), (227), (100), (41), (39), (39), (30), (27), (19), (18), (12), (12), (8), (8), (7), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.5 mg
Shipping to United States (Change)
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Request Want additional data for this product?

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Catalog No. ABIN181026
489.50 $
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Immunogen Human IL-10 expressed by COS cells. Genename: IL10
Clone JES3-12G8
Isotype IgG2a
Characteristics Synonyms: IL-10, CSIF, TGIF, Cytokine synthesis inhibitory factor
Purification Affinity Chromatography on Protein G
Alternative Name Interleukin-10 / IL10
Background IL-10 is expressed by activated CD8+ peripheral blood Tcells, activated T-helper CD4+ T cellclones mast cells, B cell lymphomas and LPS activated monocytes. This clone is reportedto neutralise the effects of IL-10.
UniProt P22301
Application Notes ELISA. Western Blot (1/100-1/250). Flow Cytometry: Use 10 µl of the working dilution to label 1x10e6 cells in 100 µl (Membranepermeabilisation is required). Immunohistochemistry on Paraffin Sections (1/100): This product does not require proteindigestion pre-treatment of paraffin sections. Enzyme pretreatment using pronase or heattreatment using 0.01M citrate buffer pH6 can be used. Recommended Positive Control: Human Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Interleukin 10 (IL10) antibody anti-Interleukin 10 (IL10) antibody
Background publications Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Burdin, Péronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed).

Gomes, Bahia-Oliveira, Rocha et al.: "Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response." in: Infection and immunity, Vol. 71, Issue 3, pp. 1185-93, 2003 (PubMed).

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