Cullin 1 (CUL1) antibody
| Antigen |
|
| Synonyms |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunoprecipitation (IP) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (6), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
|
| Catalog no. |
ABIN181295 |
| Quantity |
0.5 mg (1 mg/ml) (Variants) |
| Price |
550.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Characteristics
|
Synonyms: Cullin-1, CUL-1, CUL1
|
|
Alternative name
|
Cullin 1
|
|
Swiss-Prot
|
Q13616
|
|
Immunogen
|
A peptide from the C-terminus of the human Cullin-1.
|
|
Description
|
CUL-1 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a,-4b, and -5)homologous to the S. cerevisiae cdc53 gene. CUL 1 forms a complex with human p19skp1and F box protein p45skp2, both of which, together with cdc53 (known as CULA), as threesubunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins.
|
|
Specificity
|
Highly specific for Cullin 1. Does not react with other Cullins. Cellular Localization: Nuclear. Mol. Wt. of Antigen: 86kD. Species: Human and Mouse. Does not react with Rat. Others not tested.
|
|
Application Notes
|
Immunoprecipitation (Denaturated)(Use Protein A): Ab at 10 micrograms/mg protein lysate. Western Blotting: Ab 5-10 micrograms/ml for 2hrs at RT. Immunohistology(Formalin/paraffin): Ab 5-10 micrograms/ml for 30 min at RT (Staining of formalin-fixedtissues REQUIRES boilingtissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for20 min. ) Recommended positive control: HeLa Cells, Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
|
|
Concentration
|
1 mg/ml
|
|
Buffer
|
10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSAas stabilizer.
|
|
Storage
|
Store at 4°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch.
|
|
Research Area
|
Apoptosis/Necrosis, Proteasome
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
|
Hosts
|
Rabbit (33)
|
|
Reactivities
|
Human (30), Mouse (Murine) (24), Rat (Rattus) (18), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1), Pig (Porcine) (1)
|
|
Applications
|
Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunoprecipitation (IP) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), ELISA (6), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
C-Term (2), C-Term AA 766-776 (1)
|
