Primase, DNA, Polypeptide 1 (49kDa) (PRIM1) antibody

Details for Product No. ABIN181300
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Antigen
Reactivity
Human, Mouse (Murine)
(24), (21), (16), (1), (1)
Host
Rabbit
(25), (3)
Clonality
Polyclonal
Application
Western Blotting (WB)
(28), (17), (3), (1)
Pubmed 2 references available
Quantity 0.5 mg
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Availability Discontinued
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Catalog No. ABIN181300
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Immunogen Purified MOUSE recombinant p49 subunit of DNA primase.
Specificity This antibody is raised against the p49 subunit of DNA primase and it shows no cross reaction either with the p58 subunit of DNA primase or the DNA polymerase gamma. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs .
Purification Protein A affinity purified immunoglobulin fraction
Alternative Name DNA primase small subunit
Background In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers. The eukaryotic primase is composed of two subunits, p49 and p58, which purify as a complex tightly bound to DNA polymerase alpha. Primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer-template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. Residue 104-111 are most critical for primer synthesis. Alanine substitution in residues Glu, Asp, and These residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases.
Alternate names: DNA primase 49 kDa subunit, PRIM1
Cellular Localization: Nuclear.
Molecular Weight 49kD.
Gene ID 5557
NCBI Accession NP_000937
UniProt P49642
Application Notes Western Blot (see Ref.1). Recommended positive control: HeLa Cells. Other applicationsnot tested. Optimal dilutions of this antibody are dependent on conditions and should bedetermined by the user. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store at 2-8°C for up to one month or in aliquots at -20°C for longer.
Expiry Date 12 months
Background publications Ropp, Copeland: "Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase gamma." in: Genomics, Vol. 36, Issue 3, pp. 449-58, 1997 (PubMed).

Copeland: "Expression, purification, and characterization of the two human primase subunits and truncated complexes from Escherichia coli." in: Protein expression and purification, Vol. 9, Issue 1, pp. 1-9, 1997 (PubMed).

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