Amphiregulin (AREG) antibody
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| Synonyms |
AR, SDGF, CRDGF, MGC13647, Sdgf, AREG, AREGB, MGC152310 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN181313 |
| Quantity |
0.5 mg |
| Price |
530.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: CRDGF, AREG, SDGF, Colorectum cell-derived growth factor, Schwannoma-Derived GrowthFactor
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Alternative name
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Amphiregulin
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Swiss-Prot
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P15514
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Immunogen
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A synthetic 19-mer peptide, corresponding to aa 8-26 (VKPPQNKTESENTSDKPKR) from thesecreted amphiregulin of human origin.
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Description
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Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The maturesecreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator,which is generated by proteolytic processing of a 252-amino acid transmembraneprecursor. Seven different polypeptide ligands, which derive from distinct genes, arecapable of binding to the extracellular domain of EGFR. These ligands include EGF,TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factorscontain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteineresidues that generate 3 loops through the formation of disulfide bonds. AR protein hasbeen localized by immunohistochemistry to the epithelium of the colon, stomach,pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers ofbreast, colon, stomach, and pancreas.
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Specificity
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Highly specific for Amphiregulin. Cellular Localization: Cytoplasmic and/or nuclear. Mol. Wt. of Antigen: 18. 5-22. 5kDa. Species: Human and Mouse. Others not tested.
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Application Notes
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Immunoprecipitation (Native): use Protein A and Ab at 10 g/mg protein lysate. PositiveControl: LS174T cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
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Storage
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Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch.
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Research Area
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Cancer, Growth Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Amphiregulin (AREG)", type "Antibodies"
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Hosts
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Rabbit (21), Mouse (2)
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Reactivities
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Human (23), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (17), Horse (Equine) (17), Dog (Canine) (16), Rabbit (16), Sheep (Ovine) (16), Pig (Porcine) (3)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (5), ELISA (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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AA 8-26 (1)
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Alternatives 
