FAK antibody (PTK2 Protein tyrosine Kinase 2) (C-Term)

Details for Product anti-PTK2 Antibody No. ABIN181318, Supplier: Log in to see
Antigen
  • FADK
  • FAK
  • FAK1
  • FRNK
  • PPP1R71
  • p125FAK
  • pp125FAK
  • Fadk
  • mKIAA4203
  • PTK2 protein tyrosine kinase 2
  • protein tyrosine kinase 2
  • PTK2
  • Ptk2
  • ptk2
Alternatives
anti-Human FAK antibody for Immunofluorescence (fixed cells)
Epitope
C-Term, AA 853-1052
76
63
61
47
46
30
25
22
21
16
16
15
15
14
12
10
9
8
8
8
7
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
674
456
422
37
23
20
11
10
10
10
9
8
6
5
4
3
3
3
3
2
1
1
Host
Rabbit
590
109
3
2
1
Clonality
Polyclonal
Conjugate
This FAK antibody is un-conjugated
17
17
15
12
12
11
11
11
11
11
11
11
10
10
3
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
479
189
148
142
131
111
60
28
18
7
6
5
5
3
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK.
Specificity Reacts with FAK from severals species.
Cellular Localization: Cytoplasmic, Focal Contacts. Mol. Wt. of Antigen: 125 kDa.
Purification Protein A affinity purified immunoglobulin fraction
Alternative Name FAK1 / PTK2 (PTK2 Antibody Abstract)
Background FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.Synonyms: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK
Molecular Weight 125kD.
Gene ID 5747
NCBI Accession NP_722560
UniProt Q05397
Pathways Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Immunofluorescence. Western Blotting: Ab 5-10 μg/mL for 2hrs at RT. Recommendedpositive control: HUVEC or IMR-5 Cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09 % sodium azide as preservative and 0.2 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer.
Background publications Hanks, Polte: "Signaling through focal adhesion kinase." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 19, Issue 2, pp. 137-45, 1997 (PubMed).

Ili?, Damsky, Yamamoto: "Focal adhesion kinase: at the crossroads of signal transduction." in: Journal of cell science, Vol. 110 ( Pt 4), pp. 401-7, 1997 (PubMed).