PTK2 Protein tyrosine Kinase 2 (PTK2) (C-Term), (AA 853-1052) antibody

Details for Product No. ABIN181318
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Antigen
Synonyms FAK, FADK, FRNK, Fadk, mKIAA4203, p125FAK, FAK1, PPP1R71, pp125FAK
Epitope
C-Term, AA 853-1052
(60), (56), (54), (50), (40), (20), (20), (14), (14), (13), (12), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Frog, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(532), (352), (350), (127), (109), (108), (2), (2), (1)
Host
Rabbit
(489), (63)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (13), (13), (13), (13), (13), (13), (13), (13), (13), (13)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(372), (185), (130), (121), (96), (71), (34), (30), (9), (3), (2), (2), (1)
Pubmed 2 references available
Catalog no. ABIN181318
Quantity 0.5 mg
Price
624.36 $   Plus shipping costs $45.00
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Availability Discontinued
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Immunogen Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK.
Specificity Reacts with FAK from severals species.
Purification Protein A affinity purified immunoglobulin fraction
Alternative Name FAK1 / PTK2
Background FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non- receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
Alternate names: FADK1, FAK, Focal adhesion kinase 1, Protein-tyrosine kinase 2, pp125FAK
Cellular Localization: Cytoplasmic, Focal Contacts
Molecular Weight 125kD.
Gene ID 5747
NCBI Accession NP_722560.1
UniProt Q05397
Application Notes Immunofluorescence. Western Blotting: Ab 5-10 ug/ml for 2hrs at RT. Recommendedpositive control: HUVEC or IMR-5 Cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Preservative Sodium azide
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store at 2-8°C for up to one month or in aliquots at -20°C for longer.
Expiry Date 12 months
Background publications Hanks, Polte: "Signaling through focal adhesion kinase." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 19, Issue 2, pp. 137-45, 1997 (PubMed).

Ilić, Damsky, Yamamoto: "Focal adhesion kinase: at the crossroads of signal transduction." in: Journal of cell science, Vol. 110 ( Pt 4), pp. 401-7, 1997 (PubMed).

Hosts (489), (63)
Reactivities (532), (352), (350), (127), (109), (108), (2), (2), (1)
Applications (372), (185), (130), (121), (96), (71), (34), (30), (9), (3), (2), (2), (1)
Conjugates (13), (13), (13), (13), (13), (13), (13), (13), (13), (13), (13)
Epitopes (60), (56), (54), (50), (40), (20), (20), (14), (14), (13), (12), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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