anti-Caspase 7 antibody (Caspase 7, Apoptosis-Related Cysteine Peptidase) (N-Term)

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Antigen
Alternatives
Epitope
N-Term
(37), (29), (14), (13), (8), (6), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(263), (95), (65), (8), (7), (5), (4), (4), (3), (1), (1), (1)
Host
Rabbit
(217), (56), (4)
Clonality
Polyclonal
Conjugate
This Caspase 7 antibody is un-conjugated
(13), (10), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(228), (96), (86), (59), (32), (20), (12), (10), (10), (4), (4), (3), (2), (1)
Pubmed 2 references available
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Quantity 0.5 mg
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Immunogen A synthetic peptide from the N-terminus of human caspase 7 protein.
Specificity Reacts with Caspase 7 in western blotting.
Purification Protein A chromatography
Alternative Name Caspase-7 (CASP7 Antibody Abstract)
Background Caspase 7 functions as an effector caspase that cleaves downstream substrates responsible for producing the morphological and biochemical changes associated with apoptosis. The alpha form of Caspase 7 is 303 amino acids and gets cleaved upon activation to 20 and 12 kD subunits. A 253 aa b form, presumably a result of alternative splicing, lacks the cysteine protease active site (QACRG) and may function as a dominant negative regulator of apoptosis.
Alternate names: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3
Cellular Localization: cytoplasmic.
Molecular Weight 35kD (proform) and 20kD (cleavage fragment)
Gene ID 840
NCBI Accession NP_001218
UniProt P55210
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot: Ab 2.5-5g/ml for 2hrs at RT. Recommended positive control: Jurkat Cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10mM PBS pH 7.4 with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C or in aliquots at -20°C long term.
Expiry Date 12 months
Background publications Cardone, Salvesen, Widmann et al.: "The regulation of anoikis: MEKK-1 activation requires cleavage by caspases." in: Cell, Vol. 90, Issue 2, pp. 315-23, 1997 (PubMed).

Juan, McNiece, Argento et al.: "Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3." in: Genomics, Vol. 40, Issue 1, pp. 86-93, 1997 (PubMed).