Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody

Details for Product No. ABIN181322
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, AI115399, AW493809, CASP-9, Caspase-9, Mch6, Apaf3, Casp9_v1, Ice-Lap6, Casp-9-CTD, CASP9
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(299), (108), (94), (17), (15), (9), (7), (6), (3), (3), (1)
Host
Rabbit
(307), (30), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(285), (130), (69), (63), (51), (50), (39), (36), (10), (4), (3), (2), (1)
Pubmed 2 references available
Catalog no. ABIN181322
Quantity 0.5 mg
Price
667.92 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Discontinued
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Recombinant protein of human Caspase 9
Sequence 1-134
Specificity This antibody reacts with Caspase 9.
Purification Protein A Affinity Chromatography
Alternative Name Caspase-9
Background Activation of procaspase-9 by Apaf-1 in the cytochrome c/ dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c and dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, Caspase 9 can initiate a caspase cascade involving the downstream executioners Caspase 3, 6, and 7.
Alternate names: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Cellular Localization: Cytoplasmic.
Gene ID 842
NCBI Accession NP_001220.2
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Application Notes Western Blotting (2.5-5 ug/ml for 2hrs at RT). Immunoprecipitation (10g/mg protein lysate, Denatured verified, Use Protein A). Immunohistology (Formalin/paraffin, 5-10g/ml for 30 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended positive control: Jurkat Cells, Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09% Sodium Azide as preservative and 0.2% BSA as stabilizer
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Hakem, Hakem, Duncan et al.: "Differential requirement for caspase 9 in apoptotic pathways in vivo." in: Cell, Vol. 94, Issue 3, pp. 339-52, 1998 (PubMed).

Hosts (307), (30), (1)
Reactivities (299), (108), (94), (17), (15), (9), (7), (6), (3), (3), (1)
Applications (285), (130), (69), (63), (51), (50), (39), (36), (10), (4), (3), (2), (1)
Conjugates (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (25), (22), (18), (15), (13), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
back to top