anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP9 Antibody No. ABIN181322
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Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(428), (124), (121), (14), (12), (9), (6), (4), (3), (3), (1), (1), (1), (1)
(440), (47), (1)
This Caspase 9 antibody is un-conjugated
(13), (11), (8), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(421), (188), (129), (75), (73), (59), (50), (35), (14), (12), (5), (4), (3), (2), (1)
Pubmed 2 references available
Quantity 0.5 mg
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Immunogen Recombinant protein of human Caspase 9
Sequence 1-134
Specificity This antibody reacts with Caspase 9.
Cellular Localization: Cytoplasmic.
Purification Protein A Affinity Chromatography
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Activation of procaspase-9 by Apaf-1 in the cytochrome c/ dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c and dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, Caspase 9 can initiate a caspase cascade involving the downstream executioners Caspase 3, 6, and 7.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
NCBI Accession NP_001220
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Pathway, RTK signaling, Apoptosis Pathway
Application Notes Western Blotting (2.5-5 μg/mL for 2hrs at RT). Immunoprecipitation (10g/mg protein lysate, Denatured verified, Use Protein A). Immunohistology (Formalin/paraffin, 5-10g/mL for 30 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended positive control: Jurkat Cells, Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Hakem, Hakem, Duncan et al.: "Differential requirement for caspase 9 in apoptotic pathways in vivo." in: Cell, Vol. 94, Issue 3, pp. 339-52, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

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Catalog No. ABIN181322
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