Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) antibody

Details for Product No. ABIN181340
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Antigen
Reactivity
Human
(152), (50), (50), (18), (4), (2), (1), (1)
Host
Rabbit
(130), (27), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(126), (68), (40), (40), (27), (12), (11), (10), (8), (7), (1)
Pubmed 3 references available
Quantity 0.5 mg
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Catalog No. ABIN181340
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Immunogen A synthetic peptide from the second quarter of human MMP-14 / MT1-MMP.
Specificity This antibody detects MMP-14.
Purification Protein A chromatography
Alternative Name MMP-14
Background MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3-MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT1-MMP gets activated through the membrane plasmin cascade. It binds to TIMP-2 first and then to MMP-2 forming a trimer. It cleaves the pro-MMP-2 to its active form.
Alternate names: MMP-X1, MMP14, Matrix metalloproteinase-14, Membrane-type-1 matrix metalloproteinase
Cellular Localization: Cell membrane and cytoplasmic.
Gene ID 4323
NCBI Accession NP_004986
UniProt P50281
Research Area Cancer, Extracellular Matrix, Inflammation
Application Notes Western Blotting (5-10 ug/ml for 2 hrs at RT). Immunohistochemistry on formalin-fixed, paraffin-embedded tissues (5-10 ug/ml for 30min at RT, No special pretreatment is required). Recommended positive control: Recombinant MMP-14 Protein, Placenta. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.
Expiry Date 12 months
Supplier Images
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) antibody anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) antibody
Background publications Will, Hinzmann: "cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment." in: European journal of biochemistry / FEBS, Vol. 231, Issue 3, pp. 602-8, 1995 (PubMed).

Sato, Takino, Okada et al.: "A matrix metalloproteinase expressed on the surface of invasive tumour cells." in: Nature, Vol. 370, Issue 6484, pp. 61-5, 1994 (PubMed).

Sato, Kinoshita, Takino et al.: "Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinases (TIMP)-2." in: FEBS letters, Vol. 393, Issue 1, pp. 101-4, 1996 (PubMed).

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