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Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (Catalytic Domain) antibody

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Catalytic Domain
(32), (19), (15), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(106), (29), (28), (2), (2), (2), (1), (1), (1)
(103), (2), (1)
(6), (6), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(67), (50), (47), (26), (13), (10), (4), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.5 mg
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Immunogen A synthetic peptide from the catalytic domain of human MMP-16 / MT3-MMP.
Specificity This antibody detects MMP-16.
Purification Protein A chromatography
Alternative Name MMP-16 (MMP16 Antibody Abstract)
Background MMPs are a group of enzymes involved in matrix degradation. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT-MMPs exist as integral membrane proteins. MT1-MMP, MT2-MMP, MT3- MMP also known as MMP14, MMP15, MMP16 respectively contain a C-terminal transmembrane domain anchoring them to the cell surface. They have an 8 amino acid insert in their catalytic domain. MT1-MMP cleaves progelatinase A (MMP-2) and progelatinase B to their active forms. MT1-MMP gets activated through the membrane plasmin cascade. It binds to TIMP-2 first and then to MMP-2 forming a trimer. It cleaves the pro-MMP-2 to its active form. MT3-MMP may play a minor role in activation of pro-MMP-2 to its active form.
Alternate names: MMP-X2, MMP16, MMPX2, Matrix metalloproteinase-16, Membrane-type-3 matrix metalloproteinase
Cellular Localization: Cell membrane and cytoplasmic
Gene ID 4325
NCBI Accession NP_005932
UniProt P51512
Research Area Extracellular Matrix
Application Notes Immunohistology (formalin/paraffin: 10-20g/ml for 30 min at RT, Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min, Recommended positive control: Placenta). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.
Expiry Date 12 months
Supplier Images
Image no. 1 for anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (Catalytic Domain) antibody (ABIN181342) anti-Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16) (Catalytic Domain) antibody
Background publications Ueno, Nakamura, Inoue et al.: "Expression and tissue localization of membrane-types 1, 2, and 3 matrix metalloproteinases in human invasive breast carcinomas." in: Cancer research, Vol. 57, Issue 10, pp. 2055-60, 1997 (PubMed).

Sato, Takino, Okada et al.: "A matrix metalloproteinase expressed on the surface of invasive tumour cells." in: Nature, Vol. 370, Issue 6484, pp. 61-5, 1994 (PubMed).

Strongin, Collier, Bannikov et al.: "Mechanism of cell surface activation of 72-kDa type IV collagenase. Isolation of the activated form of the membrane metalloprotease." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5331-8, 1995 (PubMed).

Takino, Sato, Shinagawa et al.: "Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library. MT-MMPs form a unique membrane-type subclass in the MMP family." in: The Journal of biological chemistry, Vol. 270, Issue 39, pp. 23013-20, 1995 (PubMed).

Catalog No. ABIN181342

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