S100A/B (S100A/B) antibody

Antigen: S100A/B (S100A/B)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN181380

Additional Information

Characteristics: Synonyms: Protein S100-B, S100 beta, S100 calcium-binding protein B, S-100 protein subunit beta,S-100 protein beta chain, Astrocyte Marker

Alternative name: S100B (+ S100A)

Swiss-Prot: P02638

Immunogen: S100 protein purified from cow brain. Remarks: Mol. Wt. of Antigen: ~22 kDa (non-reduced), ~11 kDa (reduced)

Format: Serum

Description: S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two betachains. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost allbenign and malignant melanomas and their metastases. S100 protein is also expressed inthe antigen presenting cells such as the Langerhans cells in skin and interdigitatingreticulum cells in the paracortex of lymph nodes.

Specificity: This antibody is reactive with S100A and S100B. Cellular Localization: Cytoplasmic. Species: Human, Cow, Kangaroo, Mouse, Rat, and Chicken. Others not tested.

Application Details

Application Notes: ImmunocytochemistryImmunohistology (Formalin/paraffin: use Ab at 1: 100-1: 200 for 30 min at RT, no specialpretreatment is required for histochemical staining of formalin/paraffin tissues). Positive Control: Melanoma or Schwannoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.

Storage: Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch. Caution: S100 protein is highly soluble and may be eluted from frozen tissue during staining.

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human melanoma stained with S-100 (ABIN181380 using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of tumor cells.