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Primase, DNA, Polypeptide 2 (58kDa) (PRIM2) antibody

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Human, Mouse (Murine)
(36), (6), (5), (1), (1)
(34), (3)
(2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(32), (23)
Pubmed 2 references available
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Quantity 0.5 mg
Shipping to United States ( )
Immunogen Purified mouse recombinant p58 subunit of DNA primase.1
Specificity This antibody detects DNA Primase p58. It shows no cross reaction either with the p49 subunit of DNA primase or the DNA polymerase gamma.2 The human primase subunits are 90% identical in amino acid sequence to the mouse homologs.
Purification Protein A chromatography
Alternative Name DNA primase large subunit (PRIM2 Antibody Abstract)
Background In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers. The eukaryotic primase is composed of two subunits, p49 and p58, which purify as a complex tightly bound to DNA polymerase alpha. Primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primertemplate is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. Residue 104-111 are most critical for primer synthesis. Alanine substitution in residues Glu105, Asp109, and These residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases.
Alternate names: DNA primase 58 kDa subunit, PRIM2, PRIM2A
Cellular Localization: Nuclear
Gene ID 5558
NCBI Accession NP_000938
UniProt P49643
Pathways Telomere Maintenance
Application Notes Western Blotting: Ab 5 ug/ml for 2 hrs at RT. Mol. Wt. of Antigen: 58 kDaOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer.
Expiry Date 12 months
Background publications Copeland: "Expression, purification, and characterization of the two human primase subunits and truncated complexes from Escherichia coli." in: Protein expression and purification, Vol. 9, Issue 1, pp. 1-9, 1997 (PubMed).

Ropp, Copeland: "Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase gamma." in: Genomics, Vol. 36, Issue 3, pp. 449-58, 1997 (PubMed).

Catalog No. ABIN181387

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