Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Antigen: Baculoviral IAP Repeat-Containing 5 (BIRC5)

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN181412

Additional Information

Characteristics: Synonyms: Survivin, Apoptosis inhibitor 4, API4, Baculoviral IAP repeat-containing protein 5,Apoptosis inhibitor survivin

Alternative name: IAP4 / BIRC5

Swiss-Prot: O15392

Immunogen: A synthetic peptide derived from the C-terminus of survivinRemarks: Mol. Wt. of Antigen: 16. 5 kDa

Description: Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of thecell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates withmicrotubules of the mitotic spindle in a specific and saturable reaction that is regulated bymicrotubule dynamics. Disruption of survivin-microtubule interactions results in loss ofsurvivin's antiapoptosis function and increased caspase-3 activity, a mechanism involvedin cell death, during mitosis. It indicates that survivin may counteract a default inductionof apoptosis in G2/M phase. The overexpression of survivin in cancer may overcome thisapoptotic checkpoint and favour aberrant progression of transformed cells throughmitosis.

Specificity: This antibody detects Survivin. Species: Human, Mouse. Others not tested. Add. Information: Cellular Localization: Cytoplasmic

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Application Details

Application Notes: Immunohistology (formalin/paraffin): at 1: 500 for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: Stomach. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide

Storage: Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, Apoptosis/Necrosis, Neurology

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human stomach stained with Survivin (ABIN181412) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells in the gastric glands.