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IL-15 antibody (Biotin)

IL15 Reactivity: Mouse WB, EIA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN181593
  • Target See all IL-15 (IL15) Antibodies
    IL-15 (IL15) (Interleukin 15 (IL15))
    Reactivity
    • 58
    • 22
    • 10
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 68
    • 21
    • 4
    • 3
    • 3
    • 1
    Rabbit
    Clonality
    • 77
    • 23
    Polyclonal
    Conjugate
    • 62
    • 17
    • 8
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-15 antibody is conjugated to Biotin
    Application
    • 71
    • 36
    • 35
    • 18
    • 17
    • 10
    • 10
    • 9
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects Interleukin-15.
    Purification
    Affinity Chromatography
    Immunogen
    Highly pure (> 98%) recombinant Murine IL-15
    Top Product
    Discover our top product IL15 Primary Antibody
  • Application Notes
    Direct ELISA: To detect Murine IL-15 by Direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well ofrecombinant Murine IL-15. Sandwich ELISA: To detect Murine IL-15 by Sandwich ELISA (using 100 μL/well antibody
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2 without preservatives
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
    Expiry Date
    6 months
  • Target
    IL-15 (IL15) (Interleukin 15 (IL15))
    Alternative Name
    Interleukin-15 / IL15 (IL15 Products)
    Synonyms
    IL15 antibody, AI503618 antibody, IL-15 antibody, interleukin 15 antibody, IL15 antibody, Il15 antibody
    Background
    IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15
    Gene ID
    16168
    NCBI Accession
    NP_032383
    UniProt
    P48346
    Pathways
    JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
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