BUB1A (BUB1A) antibody
| Antigen |
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| Synonyms |
C86192, AI323730, AL022789, Ab2-098, zgc:92230, MGC127743 |
| Clonality |
Monoclonal (14H5) |
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Host
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Reactivity
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Application
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 Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN181741 |
| Quantity |
50 µg (1.0 mg/ml) (Variants) |
| Price |
470.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1
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Alternative name
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BUB1A
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Swiss-Prot
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O43683
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Immunogen
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Recombinant protein corresponding to amino acids 281-419 of human Bub1 protein. Spleencells from immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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14H5
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Description
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Bub1 cooperates with Aurora B kinase to inhibit Cdc20-mediated activation of theanaphase promoting complex/cyclosome (APC/C) until all the chromosomes correctly alignon the microtubule spindle apparatus via their kinetochores, thus ensuring high fidelitymitotic chromosome segregation. Bub1 is also required for assembly of a functional innercentromere, sister chromatid cohesion via targeting of the Shugoshin protein, andmetaphase congression. Mutation or aberrant Bub1 expression is associated withchromosomal instability, aneuploidy, and human cancer.
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Specificity
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This antibody detects Bub1, a protein kinase involved in spindle checkpoint functionduring mitosis. Species: Human. Others not tested.
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Application Notes
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Immunoprecipitation: 1: 200. Western Blot: 1: 500 - 1: 2000, detects a band of approximately 160 kDa in HeLa cell lysates. Immunofluorescence: 1: 500 - 1: 2000 (use cells grown on cover slips fixed with 3.5%paraformaldehyde in PBS at pH6.8, or 100% methanol at -20C. Permeabilise cells with0.5% Triron X-100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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PBS, pH 7.2, containing 0.01% Sodium Azide
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "BUB1A (BUB1A)", type "Antibodies"
