This monoclonal FA-11 antibody does detect surface Macrosialin at low levels in resident Mouse peritoneal Macrophages which can be enhanced with thioglycollate stimulation.
Cross-Reactivity (Details)
Species reactivity (tested):Mouse.
Purification
Affinity Chromatography on Protein G
Immunogen
Purified Concanavalin A acceptor glycoprotein from P815 cell line.
CD68
Reactivity: Human
FACS
Host: Mouse
Monoclonal
Y1-82A
PE
Application Notes
Western Blot. Immunoprecipiation. Immunohistochemistry on Frozen Sections. Flow Cytometry: 1/50-1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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Zimmermann, Homann, Bangert, Müller, Hristov, Goeser, Wiehe, Zittrich, Rottbauer, Torzewski, Pfitzer, Katus, Kaya: "Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis." in: Journal of the American Heart Association, Vol. 1, Issue 6, pp. e003293, (2013) (PubMed).
Kirby, Beattie, Maroof, van Rooijen, Kaye: "SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1107-15, (2009) (PubMed).
The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin