V5 Epitope Tag antibody

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Antigen
Reactivity
Tag
404
3
3
3
3
Host
Mouse
220
135
37
9
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
29
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
342
266
122
91
70
65
53
35
27
13
8
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Sequence GKPIPNPLLG LDST
Clone SV5-PK1
Isotype IgG2a
Purification Affinity chromatography on Protein G
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Background The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Research Area Tags/Labels
Application Notes Immunoprecipitation. Radioimmunoassasy. Immunohistochemistry on frozen sections. ELISA: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Liebau, Höpker, Müller, Schmedding, Zank, Schairer, Fabretti, Höhne, Bartram, Dafinger, Hackl, Burst, Habbig, Zentgraf, Blaukat, Walz, Benzing, Schermer: "Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia." in: The Journal of biological chemistry, Vol. 286, Issue 16, pp. 14237-45, 2011 (PubMed).

Zhang, Yu, Liebeskind: "N-amidation by copper-mediated cross-coupling of organostannanes or boronic acids with o-acetyl hydroxamic acids." in: Organic letters, Vol. 10, Issue 14, pp. 3005-8, 2008 (PubMed).

Sanchez Garcia, Ciufo, Yang, Kearsey, MacNeill: "The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit." in: Nucleic acids research, Vol. 32, Issue 10, pp. 3005-16, 2004 (PubMed).

Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, 2001 (PubMed).

Dunn, ODowd, Randall: "Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 141-50, 1999 (PubMed).

Hanke, Young, Doyle, Jones, Randall: "Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding." in: Journal of virological methods, Vol. 53, Issue 1, pp. 149-56, 1995 (PubMed).

Jaffray, Wood, Hay: "Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65." in: Molecular and cellular biology, Vol. 15, Issue 4, pp. 2166-72, 1995 (PubMed).

Rodriguez, Michalopoulos, Arenzana-Seisdedos, Hay: "Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2413-9, 1995 (PubMed).

Arenzana-Seisdedos, Thompson, Rodriguez, Bachelerie, Thomas, Hay: "Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA-binding and transcriptional activities of NF-kappa B." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2689-96, 1995 (PubMed).

Hirst, Fisher, McAndrew, Goding: "The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal." in: The EMBO journal, Vol. 13, Issue 22, pp. 5410-20, 1994 (PubMed).

Randall, Hanke, Young, Southern: "Two-tag purification of recombinant proteins for the construction of solid matrix-antibody-antigen (SMAA) complexes as vaccines." in: Vaccine, Vol. 11, Issue 12, pp. 1247-52, 1994 (PubMed).

Randall, Young, Hanke, Szawlowski, Botting: "Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming." in: Vaccine, Vol. 12, Issue 4, pp. 351-8, 1994 (PubMed).

Hanke, Szawlowski, Randall: "Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen." in: The Journal of general virology, Vol. 73 ( Pt 3), pp. 653-60, 1992 (PubMed).