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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (Feb 35)
Host
Alternatives

Mouse
Reactivity
Alternatives

Chicken
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
Catalog no. ABIN181938
Quantity 0.25 mg  (1.0 mg/ml)
Price 490.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Alternative name CD4
Immunogen Chicken embryonic thymocytes. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
Isotype IgG2b  (Matching secondary antibodies)
Clone Feb 35
Specificity This antibody recognises the homologue of human CD4, a 64kD cell surface protein expressed by thymocytes and a subset of T cells.

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (444), Rat (208), Rabbit (68)
Reactivities Human (321), Mouse (Murine) (239), Rat (Rattus) (106), Dog (Canine) (44), Cow (Bovine) (34), Chicken (33), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (601), Immunohistochemistry (Frozen Sections) (IHC (fro)) (148), Immunoprecipitation (IP) (92), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (35), Immunohistochemistry (IHC) (29), Immunocytochemistry (ICC) (16), Blocking Antibody (Inhibition) (11), Cytotoxicity Test (CyTox) (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)