NFYA antibody (C-Term)

Catalog No. ABIN182353

Product Details anti-NFYA Antibody

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: YLHESRHRHA MARKRGEGGR FFSPKEKDSP HMQDPNQADE EAMTQIIRVS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NFYA. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NFYA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 347 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYA

Target: NFYA (Nuclear Transcription Factor Y, alpha (NFYA))

Alternative Name: NFYA (NFYA Products)

Synonyms: wu:fb16e03 antibody, ik:tdsubc_2b6 antibody, xx:tdsubc_2b6 antibody, NFYA antibody, CBF-A antibody, CBF-B antibody, HAP2 antibody, NF-YA antibody, AA407810 antibody, Cbf-b antibody, SEZ-10 antibody, SEZ10 antibody, nuclear transcription factor Y, alpha antibody, nuclear transcription factor Y subunit alpha antibody, nuclear transcription factor-Y alpha antibody, nfya antibody, NFYA antibody, Nfya antibody

Background: NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
Alias Symbols: HAP2, CBF-A, CBF-B, NF-YA
Protein Interaction Partner: LUC7L2, POGZ, NFYB, GTF2A1, CSNK2A1, UBC, APPBP2, TP63, TP53, NFYC, EP300, CDK2, CCNA2, CDC25A, APP, RPS6KA6, JUN, HDAC1, ATF2, SP1, TAF6, TAF11, TAF12, PAPOLG, PWP1, SREBF2, ZHX2, ZHX1, ZHX3, KPNB1, SRSF1, HMGA1, SRF, USF2, USF1, NFYA, NR5A1, POU2F1, CEB
Protein Size: 347

Molecular Weight: 37 kDa

Gene ID: 4800

NCBI Accession: NM_002505, NP_002496

UniProt: P23511

Pathways: Regulation of Lipid Metabolism by PPARalpha