Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (C-Term) antibody

Details for Product No. ABIN182575
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Antigen
Synonyms 37B, CG18174, DmS13, Dmel\\CG18174, RPN11, RPn11, S13, p37B, rpn11, yip5, MAD1, PIG9, TP53I9, TXBP181, Mad-1, zgc:162272, PAD1, POH1, 2610312C03Rik, 3200001M20Rik, AA986732, Pad1, Poh1, rpm11
Epitope
C-Term
(13), (11), (7), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Fruit Fly (Drosophila melanogaster), Cow (Bovine), Xenopus laevis, Zebrafish (Danio rerio), Human
(74), (26), (26), (15), (9), (4), (3), (3), (3), (2), (1), (1), (1)
Host
Rabbit
(56), (21)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(49), (35), (16), (11), (10), (10), (5), (5), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN182575
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Immunogen Synthetic peptide directed towards the C terminal of human PSMD14
Sequence EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL AAMLDTVVFK
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against PSMD14. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PSMD14
Background POH1 (pad one homolog-1) is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway
Molecular Weight 35 kDa
Gene ID 10213
NCBI Accession NM_005805, NP_005796
UniProt O00487
Research Area Proteolysis / Ubiquitin, Proteasome
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 310 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (C-Term) antibody WB Suggested Anti-PSMD14 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate
anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (C-Term) antibody (2) Lanes: 1. 100 µg HeLa cell lysate2. 100 µg mouse liver cytosolic extract 3. 1.5 µg human 26S Proteasome
Primary Antibody Dilution: 1:1000
Secondary Antibody: Anti-Rabbit AP
Secondary Antibody Dilution: 1:00000
Gene Name: PSMD14

Submitted by: Anonymous
Background publications Spataro, Toda, Craig et al.: "Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit." in: The Journal of biological chemistry, Vol. 272, Issue 48, pp. 30470-5, 1997 (PubMed).

Validation Images
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