Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (N-Term) antibody

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Antigen
Epitope
N-Term
(21), (13), (12), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(95), (32), (29), (6), (5), (4), (4), (3), (2), (2), (1)
Host
Rabbit
(75), (21)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(65), (47), (20), (12), (11), (10), (8), (6), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the N terminal of human PSMD14
Sequence MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against PSMD14. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name PSMD14 (PSMD14 Antibody Abstract)
Background POH1 (pad one homolog-1) is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).
Molecular Weight 35 kDa
Gene ID 10213
NCBI Accession NM_005805, NP_005796
UniProt O00487
Research Area Proteolysis / Ubiquitin, Proteasome
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 310 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (N-Term) antibody (ABIN182576) WB Suggested Anti-PSMD14 Antibody Titration: 1.25ug/ml
ELISA Titer: ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14) (N-Term) antibody (ABIN182576) Rabbit Anti-PSMD14 Antibody ,Paraffin Embedded Tissue: Human Lung cell
Cellula...
Background publications Spataro, Toda, Craig et al.: "Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit." in: The Journal of biological chemistry, Vol. 272, Issue 48, pp. 30470-5, 1997 (PubMed).

Catalog No. ABIN182576

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