Ubiquitin Specific Peptidase 3 (USP3) (N-Term) antibody

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Antigen
Synonyms fe50h06, wu:fe50h06, USP3, SIH003, UBP, AA409661, BC017156
Epitope
N-Term
(20), (18), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(87), (10), (10), (3), (3), (3)
Host
Rabbit
(80), (7), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(66), (40), (27), (14), (11), (8), (3), (2), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human USP3
Blocking Peptide Blocking peptide for this product available: Ubiquitin Specific Peptidase 3 (USP3) peptide (ABIN985986)
Sequence GRYVNGHAKK HYEDAQVPLT NHKKSEKQDK VQHTVCMDCS SYSTYCYRCD
Predicted Reactivity Dog : 100 %, Human : 100 %, Bovine : 90 %, Horse : 90 %, African clawed frog : 90 %, Chicken : 80 %, Mouse : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against USP3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name USP3 (USP3 Antibody Abstract)
Background USP3 is a novel human ubiquitin-specific protease. The USP3 protein is a functional ubiquitin-specific protease in vitro, and is able to inhibit ubiquitin-dependent degradation of both an N-end Rule substrate and abnormal endogenous proteins in yeast. USP3 is also only the second known ubiquitin-specific protease capable of efficiently cleaving a ubiquitin-proline bond.
Molecular Weight 59 kDa
Gene ID 9960
NCBI Accession NM_006537, NP_006528
UniProt Q9Y6I4
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 520 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin Specific Peptidase 3 (USP3) (N-Term) antibody (ABIN182862) WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Background publications Puente, Sánchez, Overall et al.: "Human and mouse proteases: a comparative genomic approach." in: Nature reviews. Genetics, Vol. 4, Issue 7, pp. 544-58, 2003 (PubMed).

Catalog No. ABIN182862
$289.00
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