HIC1 antibody (AA 396-410)

Catalog No. ABIN1833970

Product Details anti-HIC1 Antibody

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Binding Specificity: AA 396-410

Reactivity: Human, Dog, Cow, Pig, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This HIC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human HIC1. This antibody is expected to recognize both reported isoforms (NP_006488.2, NP_001091672.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide HLEGYPCPHLAYGEP from an internal region of human HIC1 (NP_006488.2, NP_001091672.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Pig (100%), Guinea pig (93%), Opossum (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:128000), WB (0.5 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kD is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kD according to NP_006488.2. Recommended concentration, 0.5-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HIC1

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Alternative Name: HIC1 (HIC1 Products)

Synonyms: zfHIC1 antibody, HIC1 antibody, ZBTB29 antibody, ZNF901 antibody, hic-1 antibody, AA408311 antibody, HIC-1 antibody, hypermethylated in cancer 1 antibody, HIC ZBTB transcriptional repressor 1 antibody, hic1 antibody, HIC1 antibody, Hic1 antibody

Background: Name/Gene ID: HIC1

Synonyms: HIC1, Hic-1, ZNF901, Hypermethylated in cancer 1, ZBTB29

Gene ID: 3090

NCBI Accession: NP_006488, NP_001091672

UniProt: Q14526

Pathways: Positive Regulation of Response to DNA Damage Stimulus