ZNRF1 antibody (AA 93-106)

Catalog No. ABIN1834089

Product Details anti-ZNRF1 Antibody

Target: ZNRF1 (Zinc and Ring Finger Protein 1 (ZNRF1))

Binding Specificity: AA 93-106

Reactivity: Mouse, Cow, Guinea Pig, Dog, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This ZNRF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Specificity: Mouse ZNRF1. This antibody is expected to recognize reported isoforms a and b (NP_573469.1, NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SDSTYAHGNGYQET from an internal region of mouse ZNRF1 (NP_573469.1, NP_001162093.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Cat, Bovine, Guinea pig (100%), Rabbit, Zebra finch (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P, Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 28kD band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kD according to NP_573469.1. Recommended concentration: 1-3 μg/mL. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan IHC: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10 μg/mL. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZNRF1

Target: ZNRF1 (Zinc and Ring Finger Protein 1 (ZNRF1))

Alternative Name: ZNRF1 (ZNRF1 Products)

Synonyms: NIN283 antibody, B830022L21Rik antibody, Rnf42 antibody, Zrfp1 antibody, nin283 antibody, zgc:77896 antibody, zrfp1 antibody, zinc and ring finger 1 antibody, zinc and ring finger 1, E3 ubiquitin protein ligase S homeolog antibody, ZNRF1 antibody, Znrf1 antibody, znrf1 antibody, znrf1.S antibody

Background: Name/Gene ID: ZNRF1

Synonyms: ZNRF1, NIN283, Zinc/RING finger protein 1, Zinc and ring finger 1, Nerve injury gene 283, Zinc and ring finger protein 1

Gene ID: 84937

NCBI Accession: NP_573469, NP_001162093

UniProt: Q8ND25