DEAH (Asp-Glu-Ala-His) Box Polypeptide 16 (DHX16) (N-Term) antibody

Details for Product No. ABIN183550
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Antigen
Synonyms fa91b12, zgc:55590, wu:fa91b12, dbp2, ddx16, pro2014, prp2, prp8, prpf2, DHX16, DKFZp459L1130, DBP2, DDX16, PRO2014, PRP8, PRPF2, Prp2, 2410006N22Rik, Ddx16, mKIAA0577, Dbp2
Epitope
N-Term
(4), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Pig (Porcine), Human
(14), (3), (2)
Host
Rabbit
(9), (6)
Clonality
Polyclonal
Application
Western Blotting (WB)
(12), (4), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN183550
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human DHX16
Sequence KYQLVLEEEE TIEFVRATQL QGDEEPSAPP TSTQAQQKES IQAVRRSLPV
Predicted Reactivity Human : 100 %, Bovine : 80 %, Goat : 80 %, Pig : 80 %
Characteristics This is a rabbit polyclonal antibody against DHX16. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name DHX16
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. This gene is mapped to the MHC region on chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked.
Molecular Weight 115 kDa
Gene ID 8449
NCBI Accession NP_003578, NM_003587
UniProt O60231
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1041 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-DEAH (Asp-Glu-Ala-His) Box Polypeptide 16 (DHX16) (N-Term) antibody WB Suggested Anti-DHX16 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Validation Images
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