TAR DNA Binding Protein (TARDBP) (N-Term) antibody

Details for Product No. ABIN183734
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Antigen
Synonyms TARDBP, TDP43, DKFZp459I2127, tardbp, ALS10, TDP-43, 1190002A23Rik, C85084, Tdp43, wu:fb77f02, wu:fc52g10
Epitope
N-Term
(21), (21), (14), (13), (7), (7), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Xenopus laevis, Human, Chicken
(119), (58), (49), (22), (15), (7), (7), (6), (4), (3), (1), (1)
Host
Rabbit
(91), (29), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(108), (49), (29), (24), (24), (23), (10), (6), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN183734
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Immunogen Synthetic peptide directed towards the N terminal of human TARDBP
Sequence MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, African clawed frog : 70 %
Characteristics This is a rabbit polyclonal antibody against TARDBP. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name TARDBP
Background HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Molecular Weight 45 kDa
Gene ID 23435
NCBI Accession NP_031401, NM_007375
UniProt Q13148
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 414 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody WB Suggested Anti-TARDBP Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate
anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (2) anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (Image 2)
anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (3) anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (Image 3)
anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (4) Host: Rabbit
Target Name: TARDBP
Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1.25 µg/mL Peptide Concentration: 1.0 µg/mL Lysate Quantity: 241 µg/laneGel Concentration: 12 %TARDBP is supported by BioGPS gene expression data to be expressed in HepG2
anti-TAR DNA Binding Protein (TARDBP) (N-Term) antibody (5) WB Suggested Anti-TARDBP
Antibody Titration: 1.25 µg/mL
Positive Control: HepG2 cell lysate
TARDBP is supported by BioGPS gene expression data to be expressed in HepG2
Background publications Buratti, Brindisi, Giombi et al.: "TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 37572-84, 2005 (PubMed).

Validation Images
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