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Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (N-Term) antibody

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Antigen
Synonyms HIF-1a, HIF1a, hif-1a, hif1a, FIH1, 2310046M24Rik, A830014H24Rik, cb728, id:ibd5139
Epitope
N-Term
(24), (20), (19), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(130), (53), (31), (5), (3), (2), (2), (1)
Host
Rabbit
(86), (43)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (6), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(106), (45), (42), (32), (24), (22), (11), (6), (5), (2), (2), (2), (2)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human HIF1AN
Blocking Peptide Blocking peptide for this product available: ABIN977986
Sequence MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP
Predicted Reactivity Human : 92 %, Horse : 82 %, Rabbit : 82 %
Characteristics This is a rabbit polyclonal antibody against HIF1AN. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIF1AN (HIF1AN Antibody Abstract)
Background HIF1AN is a co-repressor that interacts with hypoxia-inducible factor 1 (HIF-1) alpha and the von Hippel-Lindau tumor suppressor protein to mediate repression of HIF-1 transcriptional activity.
Molecular Weight 40 kDa
Gene ID 55662
NCBI Accession NP_060372, NM_017902
UniProt Q9NWT6
Research Area Cancer, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 349 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (N-Term) antibody (ABIN183780) WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (N-Term) antibody (ABIN183780) WB Suggested Anti-HIF1AN
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500...
Background publications Linke, Stojkoski, Kewley et al.: "Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14391-7, 2004 (PubMed).

Catalog No. ABIN183780
$289.00
Plus shipping costs $45.00

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