Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibody

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Antigen
Epitope
C-Term
(13), (12), (11), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(57), (34), (21)
Host
Rabbit
(49), (12), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(47), (26), (20), (10), (10), (9), (3), (1), (1)
Pubmed 2 references available
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Immunogen Synthetic peptide directed towards the C terminal of human HIF3A
Sequence PGSLQDPSTP LLNLNEPLGL GPSLLSPYSD EDTTQPGGPF QPRAGSAQAD
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against HIF3A. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name HIF3A (HIF3A Antibody Abstract)
Background HIF3A is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression.The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene.
Molecular Weight 72 kDa
Gene ID 64344
NCBI Accession NM_152794, NP_690007
UniProt Q9Y2N7
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 667 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibody (ABIN183862) WB Suggested Anti-HIF3A Antibody Titration: 1.25ug/ml
Positive Control: ...
Immunohistochemistry (IHC) image for anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibody (ABIN183862) anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibody (Image 2)
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).