Keratin 18 (KRT18) (N-Term) antibody

Details for Product No. ABIN183896
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Antigen
Synonyms krt18, MGC64569, MGC75922, KRT18, CYK18, K18, CK18, Krt1-18, DreK18, cb83, dapk1, sb:cb83, wu:fa13f02, wu:fb36b04, krtt1c6, zgc:66015
Epitope
N-Term
(41), (18), (16), (12), (12), (8), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Xenopus laevis, Dog (Canine), Cow (Bovine), Human
(436), (112), (104), (43), (37), (36), (29), (12), (12), (9), (7), (6), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host
Rabbit
(253), (215), (9), (4), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(31), (28), (8), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(338), (199), (130), (129), (104), (103), (90), (72), (50), (43), (10), (4), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN183896
229.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human KRT18
Sequence TRSTFSTNYR SLGSVQAPSY GARPVSSAAS VYAGAGGSGS RISVSRSTSF
Predicted Reactivity Human : 100 %, Dog : 90 %, Bovine : 80 %
Characteristics This is a rabbit polyclonal antibody against KRT18. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name KRT18
Background KRT18 (Keratin 18) is type I intermediate filament chain. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis.KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Molecular Weight 47 kDa
Gene ID 3875
NCBI Accession NP_000215, NM_000224
UniProt P05783
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 430 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Keratin 18 (KRT18) (N-Term) antibody WB Suggested Anti-KRT18 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
anti-Keratin 18 (KRT18) (N-Term) antibody (2) anti-Keratin 18 (KRT18) (N-Term) antibody (Image 2)
anti-Keratin 18 (KRT18) (N-Term) antibody (3) Rabbit Anti-KRT18 Antibody ,Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 µg/mL
Magnification:.00X
anti-Keratin 18 (KRT18) (N-Term) antibody (4) WB Suggested Anti-KRT18
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
KRT18 is supported by BioGPS gene expression data to be expressed in HepG2
Background publications Ku, Lim, Krams et al.: "Keratins as susceptibility genes for end-stage liver disease." in: Gastroenterology, Vol. 129, Issue 3, pp. 885-93, 2005 (PubMed).

Validation Images
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