anti-Fibrillarin antibody (FBL) (N-Term)

Details for Product anti-FBL Antibody No. ABIN183938
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Antigen
Synonyms CG9888, Dmel\\CG9888, Fibri, GCR-6, GCR6, Pen59C5, fib, pen59C5, CG5725, Dmel\\CG5725, PANK, dPANK, dPANK/Fbl, FBL, MGC76139, FIB, FLRN, RNU3IP1, AL022665, wu:fb37g12, zgc:56145, zgc:77130
Epitope
N-Term
(9), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(87), (35), (30), (9), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(45), (32), (23)
Clonality
Polyclonal
Conjugate
This Fibrillarin antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(84), (33), (26), (17), (10), (7), (6), (6), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 100 μg
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Immunogen Synthetic peptide directed towards the N terminal of human FBL
Sequence GGGFHSGGNR GRGRGGKRGN QSGKNVMVEP HRHEGVFICR GKEDALVTKN
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 90 %, Bovine : 80 %
Characteristics This is a rabbit polyclonal antibody against FBL. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name FBL (FBL Antibody Abstract)
Background FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. FBL contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.
Molecular Weight 35 kDa
Gene ID 2091
NCBI Accession NM_001436, NP_001427
UniProt P22087
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 321 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Fibrillarin (FBL) (N-Term) antibody WB Suggested Anti-FBL Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
anti-Fibrillarin (FBL) (N-Term) antibody (2) anti-Fibrillarin (FBL) (N-Term) antibody (Image 2)
anti-Fibrillarin (FBL) (N-Term) antibody (3) WB Suggested Anti-FBL
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:12500
Product cited in: Armistead, Khatkar, Meyer et al.: "Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome." in: American journal of human genetics, Vol. 84, Issue 6, pp. 728-39, 2009 (PubMed).

Ernoult-Lange, Wilczynska, Harper et al.: "Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies." in: Molecular biology of the cell, Vol. 20, Issue 1, pp. 176-87, 2009 (PubMed).

Background publications Bouwmeester, Bauch, Ruffner et al.: "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. ..." in: Nature cell biology, Vol. 6, Issue 2, pp. 97-105, 2004 (PubMed).

Validation Images
Catalog No. ABIN183938
229.00 $
Plus shipping costs $45.00
Quantity
Price
100 μg
229.00 $

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