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CDC25B antibody (Cell Division Cycle 25 Homolog B (S. Pombe)) (N-Term)

Details for Product anti-CDC25B Antibody No. ABIN184115, Supplier: Login to see New
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Antigen
Synonyms CDC25B, AI604853
Alternatives
Epitope
N-Term
(27), (25), (25), (25), (22), (19), (15), (14), (13), (13), (13), (13), (13), (13), (7), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(236), (101), (83), (5), (4), (2)
Host
Rabbit
(251), (7), (2)
Clonality
Polyclonal
Conjugate
This CDC25B antibody is un-conjugated
(16), (15), (14), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(191), (108), (68), (55), (36), (20), (7), (5), (4), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human CDC25B
Blocking Peptide Blocking peptide for this product available: ABIN974724
Sequence TQTMHDLAGL GSETPKSQVG TLLFRSRSRL THLSLSRRAS ESSLSSESSE
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 90 %, Mouse : 80 %, Rat : 80 %
Characteristics This is a rabbit polyclonal antibody against CDC25B. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name CDC25B (CDC25B Antibody Abstract)
Background CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
Molecular Weight 65 kDa
Gene ID 994
NCBI Accession NP_068659, NM_021873
UniProt P30305
Research Area Cell Cycle, Cancer, Kinases/Phosphatases
Pathways Cell Division Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 580 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-CDC25B antibody (Cell Division Cycle 25 Homolog B (S. Pombe)) (N-Term) (ABIN184115) WB Suggested Anti-CDC25B Antibody Titration: 0.2-1 ug/ml
Positive Contro...
Immunohistochemistry (IHC) image for anti-CDC25B antibody (Cell Division Cycle 25 Homolog B (S. Pombe)) (N-Term) (ABIN184115) anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (N-Term) antibody (Image 2)
Background publications Mirey, Chartrain, Froment et al.: "CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 4, Issue 6, pp. 806-11, 2005 (PubMed).

Catalog No. ABIN184115
$289.00
Plus shipping costs $45.00

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