Nanog Homeobox (NANOG) (N-Term) antibody

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Antigen
Synonyms 2410002E02Rik, ENK, ecat4
Epitope
N-Term
(27), (20), (19), (15), (14), (13), (13), (12), (10), (9), (8), (7), (7), (7), (6), (5), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Pig (Porcine)
(293), (66), (35), (6), (4), (3), (2), (1)
Host
Rabbit
(203), (76), (39), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(18), (15), (15), (13), (13), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(241), (153), (81), (73), (40), (35), (32), (24), (22), (17), (15), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human NANOG
Sequence PMPVICGPEE NYPSLQMSSA EMPHTETVSP LPSSMDLLIQ DSPDSSTSPK
Cross-Reactivity Human, Pig (Porcine)
Predicted Reactivity Human : 100 %, Pig : 90 %
Purification Affinity Purified
Alternative Name NANOG (NANOG Antibody Abstract)
Background NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
Molecular Weight 35 kDa
NCBI Accession NP_079141, NM_024865
UniProt Q6NSW7
Application Notes WB Suggested Anti-NANOG Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 305 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Any unfrozen and/or unused material can be stored at 4 °C for short term use (< 1 week), and should not be re-frozen. For longer periods of storage, store at -24 °C
Supplier Images
Western Blotting (WB) image for anti-Nanog Homeobox (NANOG) (N-Term) antibody (ABIN184266) WB Suggested Anti-NANOG Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Piestun, Kochupurakkal, Jacob-Hirsch et al.: "Nanog transforms NIH3T3 cells and targets cell-type restricted genes." in: Biochemical and biophysical research communications, Vol. 343, Issue 1, pp. 279-85, 2006 (PubMed).

Catalog No. ABIN184266
$289.00
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