General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1) (C-Term) antibody

Details for Product No. ABIN184330
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Antigen
Synonyms
DDBDRAFT_0168201, DDBDRAFT_0168202, DDBDRAFT_0231096, DDB_0168201, DDB_0168202, DDB_0231096, DDBDRAFT_0217176, DDBDRAFT_0217177, DDBDRAFT_0266401, DDB_0217176, DDB_0217177, DDB_0266401, DDBDRAFT_01874 ... show more
DDBDRAFT_0168201, DDBDRAFT_0168202, DDBDRAFT_0231096, DDB_0168201, DDB_0168202, DDB_0231096, DDBDRAFT_0217176, DDBDRAFT_0217177, DDBDRAFT_0266401, DDB_0217176, DDB_0217177, DDB_0266401, DDBDRAFT_0187408, DDBDRAFT_0266450, DDB_0187408, DDB_0266450, 2610024P03Rik, 56kDa, AI303800, FE, TFIIE-A, TF2E1 show less
Epitope
C-Term
(10), (7), (4), (2), (2), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis, Fruit Fly (Drosophila melanogaster)
(27), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(18), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(22), (10), (9), (4), (4), (4), (3), (2)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN184330
289.00 $
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Immunogen Synthetic peptide directed towards the C terminal of human GTF2E1
Sequence VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE
Cross-Reactivity Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Chicken : 90 %, African clawed frog : 80 %
Purification Affinity Purified
Alternative Name GTF2E1 / TF2E1
Background The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
Molecular Weight 49 kDa
NCBI Accession NP_005504, NM_005513
UniProt P29083
Application Notes WB Suggested Anti-GTF2 E1 Antibody Titration: 0.2-1 µg/mL
Positive Control: Daudi cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 439 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Any unfrozen and/or unused material can be stored at 4 °C for short term use (< 1 week), and should not be re-frozen. For longer periods of storage, store at -24 °C
Supplier Images
anti-General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1) (C-Term) antibody WB Suggested Anti-GTF2E1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Daudi cell lysate
anti-General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1) (C-Term) antibody (2) anti-General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1) (C-Term) antibody (Image 2)
anti-General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1) (C-Term) antibody (3) Human Muscle
Background publications Ohkuma, Sumimoto, Hoffmann et al.: "Structural motifs and potential sigma homologies in the large subunit of human general transcription factor TFIIE." in: Nature, Vol. 354, Issue 6352, pp. 398-401, 1992 (PubMed).

Validation Images
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