Aurora Kinase A (AURKA) (C-Term) antibody

Details for Product No. ABIN184572
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Antigen
Synonyms
87A7-9/2, Aur-A, AurA, CG3068, Dmel\\CG3068, aur-A, aurA, ck10, ck[10], l(3)87Ac, l(3)ck10, Aurora-A, Eg2, aik, ark1, aur-a, aura, aurora2, btak, pEg2, stk15, stk6, stk7, wu:fb37c10, wu:fc02c02, zgc:1 ... show more
87A7-9/2, Aur-A, AurA, CG3068, Dmel\\CG3068, aur-A, aurA, ck10, ck[10], l(3)87Ac, l(3)ck10, Aurora-A, Eg2, aik, ark1, aur-a, aura, aurora2, btak, pEg2, stk15, stk6, stk7, wu:fb37c10, wu:fc02c02, zgc:100912, AURKA, aurka, AIK, ARK1, AURA, AURORA2, BTAK, PPP1R47, STK15, STK6, STK7, AIRK1, AU019385, AW539821, Ark1, Ayk1, IAK, IAK1, Stk6, ARK-1, aurka-a show less
Epitope
C-Term
(33), (26), (16), (7), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(170), (49), (39), (17), (15), (14), (13), (1), (1), (1), (1), (1)
Host
Goat
(128), (46), (10), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(142), (68), (50), (41), (38), (27), (17), (11), (10), (10), (4), (4), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184572
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Immunogen Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.
Specificity Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Aurora kinase A / AURKA
Background Synonyms: AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, serine/threonine protein kinase 15, serine/threonine protein kinase 6, OTTHUMP00000031340, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071
Gene ID 6790
NCBI Accession NP_003591
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45 kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8 kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Aurora Kinase A (AURKA) (C-Term) antibody ABIN184572 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zhou, Kuang, Zhong et al.: "Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation." in: Nature genetics, Vol. 20, Issue 2, pp. 189-93, 1998 (PubMed).

Validation Images
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