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Maternal Embryonic Leucine Zipper Kinase (MELK) (C-Term) antibody

Details for Product No. ABIN184576
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Antigen
Synonyms MELK, xmelk, Melk, HPK38, AI327312, MPK38, mKIAA0175, fb71b01, fe01f04, wu:fb71b01, wu:fe01f04
Epitope
C-Term
(19), (10), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(70), (18), (15), (15), (14), (12)
Host
Goat
(57), (9), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(56), (35), (10), (7), (7), (6), (6), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184576
374.27 $
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Immunogen Peptide with sequence KRLVEDILSSCKV, from the C Terminus of the protein sequence according to NP_055606.1.
Cross-Reactivity Mouse (Murine), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MELK
Background Synonyms: MELK, maternal embryonic leucine zipper kinase, KIAA0175, RP11-8N6.1, HPK38, OTTHUMP00000046113, pEg3 kinase
Gene ID 9833
NCBI Accession NP_055606.1
Research Area Embryogenesis, Embryonic stem cells, Hormones, Receptors
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Experiments gave bands at approx 75 kDa and 150 kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1 µg/mL antibody staining. The150 kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6 kDa according to NP_055606.1. Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (C-Term) antibody ABIN184576 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
General Nagase, Seki, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 1, pp. 17-24, 1996 (PubMed).

Validation Images
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