Maternal Embryonic Leucine Zipper Kinase (MELK) antibody

Antigen: Maternal Embryonic Leucine Zipper Kinase (MELK)

Synonyms: HPK38, KIAA0175, MPK38, AI327312, mKIAA0175, fb71b01, fe01f04, wu:fb71b01, wu:fe01f04, melk, MELK, Melk

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184576

Additional Information

Alternative name: MELK

Gene ID: 9833

Immunogen: KRLVEDILSSCKV

Description: All names and symbols: MELK, maternal embryonic leucine zipper kinase, KIAA0175, RP11-8N6.1, HPK38, OTTHUMP00000046113, pEg3 kinase. Official Symbol: MELK. RefSeq number(s): NP_055606.1. Gene ontology terms: protein tyrosine kinase activity, ATP binding, protein serine/threonine kinase activity, protein amino acid phosphorylation, transferase activity

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Embryogenesis, Embryonic stem cells, Hormones, Receptors

Restrictions: For Research Use only

Images

ABIN184576 (0.1µg/ml) staining of human testes lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

Publications

Nagase, Seki, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 1, pp. 17-24, 1996 (PubMed).