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Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody

Details for Product No. ABIN184593
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Antigen
Synonyms APRF, HIES, wu:fc15d02, wu:fl59g06, z-Stat3, Aprf, AW109958, 1110034C02Rik, STAT3
Epitope
C-Term
(70), (57), (42), (18), (11), (10), (9), (9), (9), (8), (7), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(366), (195), (167), (49), (47), (40), (39), (36), (15), (6), (2), (2), (2), (1)
Host
Goat
(349), (51), (12), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(16), (11), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Immunohistochemistry (IHC)
(354), (165), (139), (79), (65), (49), (45), (30), (18), (9), (7), (3), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
Shipping to United States (Change)
Availability Available
Catalog No. ABIN184593
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2, NP_644805.1.
Specificity This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
Cross-Reactivity Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name STAT3
Background Synonyms: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
Gene ID 6774, 20848, 25125
NCBI Accession NP_003141.2, NP_644805.1
Application Notes ELISA: antibody detection limit dilution 1:4000.
IHC: In paraffin embedded Mouse Thymus shows preferential staining of the medula over the cortex. Recommended concentration: 4-8 µg/mL. Immunofluorescence: An anonymous customer found positive results in IF on Human HEK 293 cells. IF, An anonymous customer found positive results in IF on Rat glial processes and nuclei.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody ABIN184593 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (2) ABIN184593 overnight staining at 10ug/ml on 4%PFA-fixed mouse thymus. Biotinylated mouse anti-goat then Streptavidin-AP then BCIP/NBT. Data kindly provided by Dr. Marella de Bruijn, MRC Mol. Haematol. Unit, WIMM, JR Hospital, Oxford.
General Bromberg, Wrzeszczynska, Devgan et al.: "Stat3 as an oncogene." in: Cell, Vol. 98, Issue 3, pp. 295-303, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN184593
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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