SMAD, Mothers Against DPP Homolog 2 (SMAD2) (Internal Region) antibody

Details for Product No. ABIN184617
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Antigen
Synonyms jv18, Xmad2, madh2, madr2, XSmad2, hmad-2, hsmad2, jv18-1, smad-2, fj43c06, wu:fj43c06, JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2
Epitope
Internal Region
(45), (38), (33), (23), (22), (18), (14), (13), (13), (12), (8), (7), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(353), (197), (191), (79), (75), (65), (13), (3), (1), (1), (1), (1)
Host
Goat
(314), (37), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(12), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(280), (143), (75), (66), (60), (52), (34), (18), (14), (9), (7), (7), (6), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184617
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence SEIWGLSTPNTIDC, from the internal region of the protein sequence according to NP_005892.1.
Cross-Reactivity Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMAD2 / MADH2
Background Synonyms: MADH2, SMAD2, MAD, mothers against decapentaplegic homolog 2 (Drosophila), JV18, MADR2, JV18-1, Mothers against decapentaplegic, Drosophila, homolog of, 2, MAD (mothers against decapentaplegic, Drosophila) homolog 2, SMAD family member 2, MGC22139, MGC34440, hMAD-2, hSMAD2, MAD, mothers against decapentaplegic homolog 2, Mad, mothers against decapentaplegic homolog 2, Mad protein homolog, Mad-related protein 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
Gene ID 4087
NCBI Accession NP_005892
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 45 kDa and 50 kDa bands seen in HeLa cell lysates. Recommended for use at 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (Internal Region) antibody ABIN184617 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).

Validation Images
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