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SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
| Antigen | SMAD, Mothers Against DPP Homolog 2 (SMAD2) |
| Synonyms | JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, MGC22139, MGC34440, SMAD2, jv18, Xmad2, madh2, madr2, XSmad2, hmad-2, hsmad2, jv18-1, smad-2 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN184617 |
| Quantity | 100 ug (Variants) |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | SMAD2 / MADH2 |
| Gene ID | 4087 |
| Immunogen | SEIWGLSTPNTIDC |
| Format | Affinity-purified |
| Description | All names and symbols: MADH2, SMAD2, MAD, mothers against decapentaplegic homolog 2 (Drosophila), JV18, MADR2, JV18-1, Mothers against decapentaplegic, Drosophila, homolog of, 2, MAD (mothers against decapentaplegic, Drosophila) homolog 2. Official Symbol: SMAD2. RefSeq number(s): NP_005892. Gene ontology terms: protein binding, regulation of transcription, DNA-dependent, signal transduction, nucleus |
| Specificity | Human |
Application Details
| Application Notes | Western blot: Approx 45kDa and 50kDa bands seen in HeLa cell lysates. Recommended for use at 1-3µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN184617 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).
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Alternatives
Alternatives for antigen "SMAD, Mothers Against DPP Homolog 2 (SMAD2)", type "Antibodies"
| Hosts | Rabbit (89), Mouse (18), Goat (7) |
| Reactivities | Human (110), Rat (Rattus) (61), Mouse (Murine) (55), Cow (Bovine) (20), Dog (Canine) (17), Rabbit (17), Chicken (16), Horse (Equine) (2), Pig (Porcine) (1) |
| Applications | Western Blotting (WB) (94), Immunofluorescence (IF) (33), Flow Cytometry (FACS) (22), Enzyme Immunoassay (EIA) (20), ELISA (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Immunohistochemistry (IHC) (15), Immunoprecipitation (IP) (7), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | pSer467 (7), pThr220 (5), S118 (2), T220 (1) |
