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SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2) (Internal Region)

Details for Product anti-SMAD2 Antibody No. ABIN184617, Supplier: Log in to see
Antigen
  • 7120426M23Rik
  • fj43c06
  • hmad-2
  • hMAD-2
  • hsmad2
  • hSMAD2
  • JV18
  • jv18
  • jv18-1
  • JV18-1
  • Madh2
  • MADH2
  • madh2
  • madr2
  • MADR2
  • Madr2
  • mMad2
  • Smad-2
  • smad-2
  • wu:fj43c06
  • Xmad2
  • XSmad2
Alternatives
anti-Human SMAD2 antibody for Western Blotting
Epitope
Internal Region
51
40
30
26
22
17
15
13
12
11
9
7
6
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
399
177
159
21
16
8
8
8
8
8
6
5
2
2
2
1
1
1
1
1
1
1
1
Host
Goat
335
53
21
Clonality
Polyclonal
Conjugate
This SMAD2 antibody is un-conjugated
14
12
10
8
8
8
2
2
2
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
332
186
82
82
47
37
27
23
19
12
6
5
5
3
3
Supplier
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Immunogen Peptide with sequence SEIWGLSTPNTIDC, from the internal region of the protein sequence according to NP_005892.1.
Blocking Peptide Blocking peptide for this product available: ABIN370444
Cross-Reactivity Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMAD2 / MADH2 (SMAD2 Antibody Abstract)
Background Synonyms: MADH2, SMAD2, MAD, mothers against decapentaplegic homolog 2 (Drosophila), JV18, MADR2, JV18-1, Mothers against decapentaplegic, Drosophila, homolog of, 2, MAD (mothers against decapentaplegic, Drosophila) homolog 2, SMAD family member 2, MGC22139, MGC34440, hMAD-2, hSMAD2, MAD, mothers against decapentaplegic homolog 2, Mad, mothers against decapentaplegic homolog 2, Mad protein homolog, Mad-related protein 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
Gene ID 4087
NCBI Accession NP_005892
Pathways Cell Division Cycle
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 45 kDa and 50 kDa bands seen in HeLa cell lysates. Recommended for use at 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-SMAD2 antibody (SMAD, Mothers Against DPP Homolog 2) (Internal Region) (ABIN184617) ABIN184617 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane...
Background publications Eppert, Scherer, Ozcelik, Pirone, Hoodless, Kim, Tsui, Bapat, Gallinger, Andrulis, Thomsen, Wrana, Attisano: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).