Sphingosine Kinase 1 (SPHK1) (C-Term) antibody

Details for Product No. ABIN184663
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Antigen
Epitope
C-Term
(21), (8), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(96), (32), (32), (15), (13), (2), (1)
Host
Goat
(96), (11), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(79), (30), (22), (14), (13), (10), (6), (4), (4), (3), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184663
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Immunogen Peptide with sequence C-SWKPQQMPPPEEPL, from the C Terminus of the protein sequence according to NP_068807.2, NP_892010.2, NP_001136073.1.
Specificity This antibody is expected to recognize all reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). The variants NP_001136073.1 and NP_001136074.1 represent identical protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SPHK1
Background Synonyms: SPHK1, sphingosine kinase 1, SPHK
Gene ID 8877
NCBI Accession NP_892010, NP_001136073, NP_068807
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in Human Lung and Placenta lysates (calculated MW of 51.1 kDa according to NP_892010.1). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Kohama, Olivera, Edsall et al.: "Molecular cloning and functional characterization of murine sphingosine kinase." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23722-8, 1998 (PubMed).

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