PYCARD antibody (PYD and CARD Domain Containing) (C-Term)

Details for Product anti-PYCARD Antibody No. ABIN184674, Supplier: Log in to see
Antigen
  • ASC
  • CARD5
  • TMS
  • TMS-1
  • TMS1
  • 9130417A21Rik
  • Asc
  • TNS1
  • masc
  • asc1
  • zAsc
  • zgc:109874
  • PYD and CARD domain containing
  • PYCARD
  • Pycard
  • pycard
Alternatives
anti-Human PYCARD antibody for ELISA
Epitope
C-Term
35
16
15
13
12
7
6
5
4
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
145
44
30
3
2
2
1
1
1
1
Host
Goat
97
36
20
Clonality
Polyclonal
Conjugate
This PYCARD antibody is un-conjugated
7
6
5
5
4
4
2
2
2
2
2
2
2
2
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
129
60
55
50
37
36
13
5
5
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.
Blocking Peptide Blocking peptide for this product available: ABIN369765
Specificity This antibody is expected to recognise all reported Human isoforms according to NP_037390.2 and NP_660183.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ASC / TMS1 (PYCARD Antibody Abstract)
Background Synonyms: PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1
Gene ID 29108
NCBI Accession NP_660183, NP_037390
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 22 kDa band observed in Hela and Human Kidney lysates (predicted MW of 22 kDa according to NP_037390.2). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-PYCARD antibody (PYD and CARD Domain Containing) (C-Term) (ABIN184674) ABIN184674 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary in...
Background publications Ohtsuka, Ryu, Minamishima, Macip, Sagara, Nakayama, Aaronson, Lee: "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway." in: Nature cell biology, Vol. 6, Issue 2, pp. 121-8, 2004 (PubMed).